SPA17 protein (His tag) (80R-2750)

Purified recombinant SPA17 protein (His tag)

Synonyms Sperm surface protein Sp17 protein, CT22 protein, SP17 protein, SP17-1 protein, SPA17, SPA 17, SPA-17
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of SPA17 protein (His tag) (80R-2750)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-151 aa: MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK
Expression System E.coli
Purity > 90% pure
Molecular Weight 19.8 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration 1 mg/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of SPA17 protein (His tag) (80R-2750) | Figure annotation denotes ug of protein loaded and % gel used.
  • SDS-PAGE analysis of SPA17 protein (His tag) (80R-2750) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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