SRGN protein (His tag) (80R-1996)

Recombinant human SRGN protein (His tag)

Synonyms FLJ12930 protein, PRG1 protein, PRG protein, MGC9289 protein, PPG protein, Serglycin precursor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of SRGN protein (His tag) (80R-1996)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 28-158 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML
Expression System E.coli
Purity > 90% pure
Molecular Weight 17.4 kDa (156aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations SRGN protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance SRGN is a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. Recombinant human SRGN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of SRGN protein (His tag) (80R-1996) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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