SSU72 protein (His tag) (80R-2143)

Recombinant human SSU72 protein (His tag)

Synonyms SSU 72, PNAS-120 protein, SSU72, HSPC182 protein, SSU-72, SSU72 RNA polymerase II CTD phosphatase homolog protein, SSU-72 protein, SSU 72 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of SSU72 protein (His tag) (80R-2143)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-194 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMPSSPLR VAVVCSSNQN RSMEAHNILS KRGFSVRSFG TGTHVKLPGP APDKPNVYDF KTTYDQMYND LLRKDKELYT QNGILHMLDR NKRIKPRPER FQNCKDLFDL ILTCEERVYD QVVEDLNSRE QETCQPVHVV NVDIQDNHEE ATLGAFLICE LCQCIQHTED MENEIDELLQ EFEEKSGRTF LHTVCFY
Expression System E.coli
Purity > 95% pure
Molecular Weight 25 kDa (217aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance SSU72 RNA polymerase II CTD phosphatase homolog, also known as SSU72, is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. Existing as multiple alternatively spliced isoforms, SSU72 interacts with TFIIB, Rb and DNAM-1 and functions to catalyze the dephosphorylation of target proteins, possibly playing a role in RNA processing and termination via dephosphorylation of Pol II. Recombinant human SSU72 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of SSU72 protein (His tag) (80R-2143) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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