STAR protein (His tag) (80R-2034)

Recombinant human STAR protein (His tag)

Synonyms Steroidogenic acute regulatory protein, mitochondrial protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of STAR protein (His tag) (80R-2034)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 64-285 amino acids: MGSSHHHHHH SSGLVPRGSH MEETLYSDQE LAYLQQGEEA MQKALGILSN QEGWKKESQQ DNGDKVMSKV VPDVGKVFRL EVVVDQPMER LYEELVERME AMGEWNPNVK EIKVLQKIGK DTFITHELAA EAAGNLVGPR DFVSVRCAKR RGSTCVLAGM ATDFGNMPEQ KGVIRAEHGP TCMVLHPLAG SPSKTKLTWL LSIDLKGWLP KSIINQVLSQ TQVDFANHLR KRLESHPASE ARC
Expression System E.coli
Purity > 90% pure
Molecular Weight 27.1 kDa (243aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations STAR protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance STAR, also known as steroidogenic acute regulatory protein, appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. It increases pregnenolone synthesis more than four-fold and a major STAR transcript of 1.6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of it tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult. Recombinant human STAR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of STAR protein (His tag) (80R-2034) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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