STAT6 antibody (10R-1225)

Mouse monoclonal STAT6 antibody

Synonyms Monoclonal STAT6 antibody, Anti-STAT6 antibody, STAT-6, STAT 6 antibody, STAT6B antibody, STAT6C antibody, STAT-6 antibody, IL-4-STAT antibody, Signal transducer and activator of transcription 6 antibody, D12S1644 antibody, STAT6, STAT 6
Specificity Human STAT6
Cross Reactivity Human. Other species not studied
Applications ELISA, FC, ICC, WB
Immunogen STAT6 antibody was raised in mouse using recombinant Signal Transducer And Activator Of Transcription 6, Interleukin-4 Induced (Stat6)

Images

Flow Cytometric assay using STAT6 antibody (10R-1225)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-STAT6 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone STAAD23A
Isotype IgG1
Method of Purification STAT6 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer STAT6 antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4 , 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

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Images

  • Flow Cytometric assay using STAT6 antibody (10R-1225) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-STAT6 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using STAT6 antibody (10R-1225)
  • Immunofluorescent staining using STAT6 antibody (10R-1225) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-STAT6 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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