STEAP1 antibody (10R-1109)

Mouse monoclonal STEAP1 antibody

Synonyms Monoclonal STEAP1 antibody, Anti-STEAP1 antibody, STEAP-1 antibody, STEAP 1, PRSS24 antibody, six transmembrane epithelial antigen of the prostate antibody, STEAP-1, six transmembrane epithelial antigen of the prostate 1 antibody, STEAP 1 antibody, STEAP1
Specificity Human
Cross Reactivity Human, Rat
Applications ELISA, IF, IHC, WB
Immunogen STEAP1 antibody was raised in mouse using recombinant human STEAP1 (1-70aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of STEAP1 antibody (10R-1109)

Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone J2D2
Isotype IgG2b kappa
Source Anti-human STEAP1 antibody, clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.
Method of Purification STEAP1 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100-1:300, IHC: 1:50-1:100, WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.

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Images

  • Western Blot analysis of STEAP1 antibody (10R-1109) | Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunofluorescent staining using STEAP1 antibody (10R-1109) | Immunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody.
  • Immunohistochemical staining using STEAP1 antibody (10R-1109) | Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Availability: In stock

Price: $265.00
Size: 100 ul
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