STK3 antibody (10R-1630)

Mouse monoclonal STK3 antibody

Synonyms Monoclonal STK3 antibody, Anti-STK3 antibody, Serine/threonine-protein kinase 3 antibody, STK 3 antibody, STK 3, FLJ90748 antibody, STK-3, STK-3 antibody, KRS1 antibody, STK3, MST2 antibody
Specificity Human STK3
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen STK3 antibody was raised in mouse using recombinant Human Serine/Threonine Kinase 3 (Ste20 Homolog, Yeast) (Stk3)

Images

Immunofluorescent staining using STK3 antibody (10R-1630)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-STK3 antibody.

Specifications

Host Mouse
Clone 3067C3a
Isotype IgG2b
Method of Purification STK3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer Mouse monoclonal anti-humanSTK3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosisinducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand.

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Images

  • Immunofluorescent staining using STK3 antibody (10R-1630) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-STK3 antibody.
  • Western Blot analysis of immunized recombinant protein using STK3 antibody (10R-1630)

Availability: In stock

Price: $190.00
Size: 50 ug
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