Streptavidin Poly-HRP Blotting Starter Kit (55R-SPBLOT)
Conjugated Streptavidin Poly HRP kit for High Sensitivity Blotting assays
Storage & Safety
|Storage||Store at 4 deg C.|
|Biological Significance||Three different Streptavidin-PolyHRP (SA-PolyHRP) conjugates have made a product line of universal reagents currently available for ultrasensitive detection in ELISA and related assay methods. SA-PolyHRP conjugates are built up from on an average five identical HRP homopolymer blocks chemically coupled to multiple streptavidin molecules distributed mainly on their outer surface. The estimated average number of HRP monomer molecules inSA-PolyHRP20 conjugate is 100 (20 X 5), in SA-PolyHRP40 - 200 (40 X 5) and in SA-PolyHRP80 - 400 (80 X 5). Thus, PolyHRP brings in reaction with substrate development system much larger number of enzyme label molecules (per one bound analyte molecule) than conventional conjugates do. In contrast to other methods, such as bridge, complex, multilayer and catalysed reporter deposition techniques, PolyHRP detection enables the achievement of much higher sensitivities in one or two (if biotin-streptavidin is used) steps, i.e. assay enhancement is set up by the design of the detecting conjugate itself.|
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