Streptavidin protein (Cy5) (65C-CE0306)
Purified homogeneous preparation of Cy5 conjugated Streptavidin protein
|Protein Type||Binding Protein|
|Applications||ELISA, FC, IF|
Western blot analysis using Streptavidin protein (Cy5) (65C-CE0306)
Western Blot of Cy5 conjugated Streptavidin. Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: none. Load: 100 ng per lane. none. Cy5 conjugated Streptavidin at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 28 kDa for Guinea Pig IgG..
|Method of Purification||Streptavidin protein (Cy5) was purified by Protein A affinity chromatography.|
|Form & Buffer||Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.|
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