Streptavidin protein (His tag) (80R-1063)

Purified recombinant Streptomyces avidinii Streptavidin protein

Synonyms Streptavidin protein, SA V2 protein, Streptomyces avidinii streptavidin protein, Streptavidin V2., Streptavidin V1 protein, SA V1 protein
Species Streptomyces avidinii
Protein Type Binding Protein
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Streptavidin protein (His tag) (80R-1063)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 25-183 amino acids: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
Expression System E.coli
Purity > 95% pure
Molecular Weight 17 kDa (167 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris, pH 7.5.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Streptavidin protein (His tag) (80R-1063) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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