Streptavidin protein (His tag) (80R-2845)

Purified recombinant Streptavidin protein (His tag)

Synonyms Streptavidin, Streptavidin, Streptavidin protein, STAV protein
Species Streptomyces avidinii
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of Streptavidin protein (His tag) (80R-2845)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 37-159 aa: MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP
Expression System E.coli
Purity > 90% pure
Molecular Weight 15.6 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of Streptavidin protein (His tag) (80R-2845) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $275.00
Size: 100 ug
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