Streptavidin protein (HRP) (65C-CE0309)

Purified homogeneous preparation of HRP conjugated Streptavidin protein

Species Streptomyces avidinii
Protein Type Binding Protein
Applications ELISA, IHC, WB

Specifications

Method of Purification Streptavidin protein (HRP) was purified by Protein A affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and rotease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:200,000, IHC: 1:1,000-1:5,000, WB: 1:10,000-1:40,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.

References
Add a Paper

Lu Y, Hoyte K, Montgomery WH, Luk W, He D, Meilandt WJ, Zuchero YJ, Atwal JK, Scearce-Levie K2, Watts RJ, DeForge LE

Characterization of a sensitive mouse Aβ40 PD biomarker assay for Alzheimer's disease drug development in wild-type mice

Bioanalysis

Volume: 8 (2016) Issue: 10 Page: 1067-1075 PMID: 27094761 DOI: 10.4155/bio-2016-0003

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Availability: In stock

Price: $250.00
Size: 1 mg
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