SUB1 protein (His tag) (80R-1337)
Purified recombinant Human SUB1 protein
|Synonyms||SUB-1, Interferon related developmental regulator 1 protein, Positive cofactor 4 protein, PC4 protein, SUB 1, SUB-1 protein, SUB1 homolog., SUB1 protein, MGC102747 protein, P15 protein, : p14 protein, Activated RNA polymerase II transcriptional coactivator p15 Activated RNA polymerase II transcriptional coactivator p15 protein, SUB1, PC4 LSB protein, RPO2TC1 protein, SUB 1 protein|
Coomassie Blue stained SDS-PAGE of SUB1 protein (His tag) (80R-1337)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-127 amino acids: MGSSHHHHHH SSGLVPRGSH MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI DDAVRKL|
|Purity||> 90% pure|
|Molecular Weight||16.5 kDa (147aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SUB1, also known as PC4, is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. This protein induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Recombinant human SUB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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