SUMF1 protein (His tag) (80R-2043)
Recombinant human SUMF1 protein (His tag)
|Synonyms||SUMF 1 protein, SUMF-1, SUMF 1, SUMF1, FGE protein, Sulfatase modifying factor 1 protein, SUMF-1 protein|
SDS-PAGE analysis of SUMF1 protein (His tag) (80R-2043)
SDS-PAGE gel analysis of SUMF1 protein (His tag) where the figure is annotated with amount of protein run on the gel.
|Residues||91-374 amino acids: MGSSHHHHHH SSGLVPRGSH MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD|
|Purity||> 85% pure|
|Molecular Weight||34.1 kDa (304aa)|
|Form & Buffer||Liquid. In 20mM Tris-HCl, pH8.0, 2M Urea, 20%Glycerol, 2mM DTT|
Usage & Assay Information
|Usage Recommendations||SUMF1 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||SUMF1 (Sulfatase-modifying factor 1) belongs to the SUMF family. SUMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant human SUMF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.|
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