SUMO2 antibody (10R-1181)

Mouse monoclonal SUMO2 antibody

Synonyms Monoclonal SUMO2 antibody, Anti-SUMO2 antibody, Sentrin-2 antibody, Sentrin2 antibody, SMT3A antibody, sumo3 antibody, SUMO2, MGC117191 antibody, Sumo3 antibody, SUMO-2 antibody, SUMO-3 antibody, HSMT3 antibody, SUMO-2, SMT3B antibody, SUMO 2 antibody, SUMO-2 antibody, sumo 3 antibody, Sumo2 antibody, SUMO-3 antibody, SUMO 2, SMT3 homolog 2 antibody, SMT 3B antibody, SMT3 suppressor of mif two 3 homolog 2 antibody, Small ubiquitin-related modifier 2 antibody, Ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3 antibody, SMT3H1 antibody, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S antibody, SMT3H2 antibody, Small ubiquitin like modifier 2 antibody, Sentrin 2 antibody, Small ubiquitin related modifier 2 antibody, HSMT 3 antibody, cerevisiae) antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen SUMO2 antibody was raised in mouse using recombinant human SUMO2 (1-93aa) purified from E.coli as the immunogen.

Images

Western Blot analysis of SUMO2 antibody (10R-1181)

Cell lysates of HeLa (35 ug) and recombinant proteins (10 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT10F1
Isotype IgG2b kappa
Source Anti-human SUMO2 antibody, clone AT10F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SUMO2 protein.
Method of Purification SUMO2 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8?11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the ?GG? region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis of SUMO2 antibody (10R-1181) | Cell lysates of HeLa (35 ug) and recombinant proteins (10 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemical staining using SUMO2 antibody (10R-1181) | Paraffin embedded sections of colorectal canitrocelluloseer tissue were initrocelluloseubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Availability: In stock

Price: $265.00
Size: 100 ul
OR
Shipping
View Our Distributors