SurA protein (His tag) (80R-1426)

Purified recombinant E.coli SurA protein

Synonyms Survival protein A protein, PPIase surA protein, Rotamase surA protein, Chaperone surA
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant SurA protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes.

Images

Coomassie Blue stained SDS-PAGE of SurA protein (His tag) (80R-1426)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 21-428 amino acids: MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN
Expression System E.coli
Purity > 95% pure
Molecular Weight 47.3 kDa(429aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 40 nM/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E. coli in stationary phase and required for pilus biogenesis. Recombinant E. coli SurA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of SurA protein (His tag) (80R-1426) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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