Surfactant Protein D antibody (20R-SR012M)

Mouse monoclonal Surfactant Protein D antibody

Synonyms Monoclonal Surfactant Protein D antibody, Anti-Surfactant Protein D antibody, Surfactant Protein D, Surfactant D antibody, Anti-Surfactant D, SP-D antibody, SP-D
Specificity Rat, Human (recognizes an epitope localized in the lectin domain of rat surfactant protein D)
Cross Reactivity Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells, SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident
Applications ELISA, IHC-F, IHC-P, WB
Immunogen Surfactant Protein D antibody was raised using purified ratSP-D from bronchoalveolar lavage

Specifications

Host Mouse
Clone IVF11
Isotype IgG1kappa
Method of Purification IgG1 purified from tissue culture supernatant
Form & Buffer Lyophilized from buffer containing 5 mg/ml BSA and 0.09% sodium azide

Usage & Assay Information

Usage Recommendations IHC:fresh rat tissue 1:400;fresh human tissue 1:20. Fix inacetone for 5 min at 4 deg, paraformaldehyde, paraffin.Pretreat paraffin tissues inmicrowavefor 2 x 5 min at 750 W. ELISA: Use as a capture antibody.

Storage & Safety

Storage Storelyophilized material at 2-8 degfor up to1 year.Reconstitutewith 500 ul sterile distilled water, aliquot andstore at -20 degfor up to 6 months, avoidingrepeated freeze/thaw cycles.

General Information

Biological Significance Surfactant protein D is a calcium dependent carbohydrate binding protein. The function of SP-D is not fully identified but it is known that SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. It has a similar structure to other C-type mammalian lectins and a molecular weight of 620 kDa (non-reducing conditions) and 43 kDa (reducing conditions).

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Availability: In stock

Price: $595.00
Size: 200 ug
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