TAF15 antibody (10R-1265)

Mouse monoclonal TAF15 antibody

Synonyms Monoclonal TAF15 antibody, Anti-TAF15 antibody, TAF-15 antibody, TATA-binding protein-associated factor 2N antibody, TAF 15, TAF15, TAF 15 antibody, TAFII68 antibody, TAF2N antibody, TAF-15, RBP56 antibody, hTAFII68 antibody
Specificity Human TAF15
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen TAF15 antibody was raised in mouse using recombinant Taf15 Rna Polymerase Ii, Tata Box Binding Protein (Tbp)-Associated Factor, 68Kda

Images

Immunofluorescent staining using TAF15 antibody (10R-1265)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-TAF15 in PBS. The cells were immunostained with anti-GATAD1 antibody.

Specifications

Host Mouse
Clone TAF15B11A6
Isotype IgG1
Method of Purification TAF15 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TAF15 antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4 140 mM NaCl, 8.0 mM Na2HPO4(pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

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Images

  • Immunofluorescent staining using TAF15 antibody (10R-1265) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-TAF15 in PBS. The cells were immunostained with anti-GATAD1 antibody.
  • Western Blot analysis of immunized recombinant protein using TAF15 antibody (10R-1265)

Availability: In stock

Price: $190.00
Size: 50 ug
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