TAF6 antibody (10R-1319)

Mouse monoclonal TAF6 antibody

Synonyms Monoclonal TAF6 antibody, Anti-TAF6 antibody, TAFII70 antibody, DKFZp781E21155 antibody, TAF2E antibody, TAF 6 antibody, TAF6, TAF 6, TAF-6 antibody, TAFII85 antibody, TAF-6, Transcription initiation factor TFIID subunit 6 antibody, MGC:8964 antibody, TAFII80 antibody
Specificity Human TAF6
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen TAF6 antibody was raised in mouse using recombinant Human Taf6 Rna Polymerase Ii, Tata Box Binding Protein (Tbp)-Associated Factor, 80Kda (Taf6)

Images

Immunofluorescent staining using TAF6 antibody (10R-1319)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TAF6 antibody.

Specifications

Host Mouse
Clone 585D4a
Isotype IgG1
Method of Purification TAF6 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TAF6 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID.

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Images

  • Immunofluorescent staining using TAF6 antibody (10R-1319) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TAF6 antibody.
  • Western Blot analysis of immunized recombinant protein using TAF6 antibody (10R-1319)

Availability: In stock

Price: $190.00
Size: 50 ug
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