TAF9L antibody (10R-1450)

Mouse monoclonal TAF9L antibody

Synonyms Monoclonal TAF9L antibody, Anti-TAF9L antibody, TAFL 9 antibody, DN-7 antibody, Transcription-associated factor TAFII31L antibody, TAFL-9, TFIID-31 antibody, DN7 antibody, TAF9L, TAFL-9 antibody, TAFII31L antibody, TAF9B antibody, TAFL 9
Specificity Human TAF9L
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen TAF9L antibody was raised in mouse using recombinant Human Taf9B Rna Polymerase Ii, Tata Box Binding Protein, (Tbp)-Associated Factor, 31Kda (Taf9B)

Images

Immunofluorescent staining using TAF9L antibody (10R-1450)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TAF9L antibody.

Specifications

Host Mouse
Clone 3365C4a
Isotype IgG2b
Method of Purification TAF9L antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TAF9L antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

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Images

  • Immunofluorescent staining using TAF9L antibody (10R-1450) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TAF9L antibody.
  • Western Blot analysis of immunized recombinant protein using TAF9L antibody (10R-1450)

Availability: In stock

Price: $190.00
Size: 50 ug
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