TARS antibody (10R-1304)

Mouse monoclonal TARS antibody

Synonyms Monoclonal TARS antibody, Anti-TARS antibody, ThrRS antibody, ThrRS antibody, Anti TARS antibody, MGC9344 antibody, TARS antibody, Threonyl-tRNA synthetase antibody, Threonyl-tRNA synthetase antibody, MGC9344 antibody
Specificity Human TARS
Cross Reactivity Human. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen TARS antibody was raised in mouse using recombinant Human Threonyl-Trna Synthetase

Images

Flow Cytometric assay using TARS antibody (10R-1304)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TARS antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone TARSF8H3
Isotype IgG2a
Method of Purification TARS antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family.

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Images

  • Flow Cytometric assay using TARS antibody (10R-1304) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TARS antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using TARS antibody (10R-1304) | Left: TARS staining. Right: Coomassie Blue staining of immunized recombinant protein.
  • Immunofluorescent staining using TARS antibody (10R-1304) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TARS antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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