TBCA antibody (10R-1152)

Mouse monoclonal TBCA antibody

Synonyms Monoclonal TBCA antibody, Anti-TBCA antibody, Tubulin folding cofactor A antibody, Co chaperonin associated with a & b tubulin antibody, Tubulin folding cofactor A CFA antibody, tubulin specific chaperone a antibody, Tubulin cofactor a. antibody, TBCA antibody, TCP1 chaperonin cofactor A antibody, Anti TBCA antibody , Tubulin cofactor a. antibody, TCP1 chaperonin cofactor A antibody, Tubulin folding cofactor A CFA antibody, tubulin specific chaperone a antibody, chaperonin cofactor a antibody, Tubulin folding cofactor A antibody, Cofactor A antibody, TBCA antibody, Co chaperonin associated with a & b tubulin antibody, chaperonin cofactor a antibody, Cofactor A antibody, TBCA antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen TBCA antibody was raised in mouse using recombinant TBCA (1-108aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of TBCA antibody (10R-1152)

Cell lysates of LnitrocelluloseaP(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT1A5
Isotype IgG2b kappa
Source Anti-human TBCA antibody, clone AT1A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TBCA protein.
Method of Purification TBCA antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

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Images

  • Western Blot analysis of TBCA antibody (10R-1152) | Cell lysates of LnitrocelluloseaP(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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