TBP antibody (10R-1278)

Mouse monoclonal TBP antibody

Synonyms Monoclonal TBP antibody, Anti-TBP antibody, MGC117320 antibody, TBP antibody, TATA box binding protein antibody, GTF2D1 antibody, MGC117320 antibody, MGC126054 antibody, Anti TBP antibody, GTF2D antibody, SCA17 antibody, SCA17 antibody, MGC126054 antibody, TATA box binding protein antibody, TFIID antibody, MGC126055 antibody, GTF2D antibody, TFIID antibody, MGC126055 antibody, GTF2D1 antibody
Specificity Human TBP
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen TBP antibody was raised in mouse using recombinant Tata Box Binding Protein

Images

Immunofluorescent staining using TBP antibody (10R-1278)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TBP antibody.

Specifications

Host Mouse
Clone TBPB8A9
Isotype IgG2b
Method of Purification TBP antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TBP antibody in PBS (3.0 mM KCl,1.5 mM KH2PO4 , 140 mM NaCl , 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein.

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Images

  • Immunofluorescent staining using TBP antibody (10R-1278) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TBP antibody.
  • Western Blot analysis of immunized recombinant protein using TBP antibody (10R-1278)

Availability: In stock

Price: $190.00
Size: 50 ug
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