TCEA1 protein (His tag) (80R-2274)
Purified recombinant Human TCEA1 Protein (His tag)
|Synonyms||TCEA1, TFIIS protein, TCEA protein, SII protein, TF2S protein, TCEA 1 protein, TCEA-1, TCEA-1 protein, GTF2S protein, TCEA 1|
Coomassie Blue stained SDS-PAGE of TCEA1 protein (His tag) (80R-2274)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC|
|Purity||> 90% pure|
|Molecular Weight||36.5 kDa|
|Form & Buffer||Supplied in liquid form in 20 mM Tris-HCl buffer, pH7.5, 20% glycerol, 1mM DTT, 50mM NaCl|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||TCEA1 belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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