TDP1 protein (His tag) (80R-2654)
Purified recombinant Human TDP1 protein (His tag)
|Synonyms||FLJ11090 protein, TDP1 protein, Tyrosyl DNA phosphodiesterase 1 protein, TDP1, TDP 1 protein, TDP-1 protein, Tyrosyl-DNA phosphodiesterase 1 protein, TDP 1, SCAN1 protein, TDP-1 protein, TDP 1 protein, TDP-1, Tyr-DNA phosphodiesterase 1 protein|
SDS-PAGE analysis of TDP1 protein (His tag) (80R-2654)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-298 aa: MGSSHHHHHH SSGLVPRGSH MSQEGDYGRW TISSSDESEE EKPKPDKPST SSLLCARQGA ANEPRYTCSE AQKAAHKRKI SPVKFSNTDS VLPPKRQKSG SQEDLGWCLS SSDDELQPEM PQKQAEKVVI KKEKDISAPN DGTAQRTENH GAPACHRLKE EEDEYETSGE GQDIWDMLDK GNPFQFYLTR VSGVKPKYNS GALHIKDILS PLFGTLVSSA QFNYCFDVDW LVKQYPPEFR KKPILLVHGD KREAKAHLHA QAKPYENISL CQAKLDIAFG THHTKMMLLL YEEGLRVVIH TSNLIHADWH QKTQGTHL|
|Method of Purification||TDP1 protein was purified using Protein G affinity chromatography|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Tyrosyl-DNA phosphodiesterase 1, also known as TDP1, is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. Recombinant human TDP1, fused to His-tag at N-terminus, was expressed in E.coli.|
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