TGF beta 1 protein (30R-AT072)

Purified recombinant Human TGF beta 1 protein

Synonyms TGF beta 1, TGF beta-1 protein, TGFB1 protein, TGF-1b protein, TGF beta 1 protein, TGF beta-1, Differentiation inhibiting factor protein, Cartilage-inducing factor protein, TGF beta 1, TGF beta-1 protein, Transforming Growth Factor-beta1 protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of TGF beta 1 protein corresponding to amino acids ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
Expression System 293 cells
Purity > 98% pure
Form & Buffer Lyophilized from 0.1% TFA. Reconstitute in 10 mM Citric Acid, pH 3.0 to a concentration of 0.1 - 1.0 mg/ml.

Usage & Assay Information

Bioactivity The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is approximately 0.05 ng/ml, corresponding to a specific activity of approximately 2 x 10^7 units/mg.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance The three mammalian isoforms of TGF beta, TGF beta1, beta2, beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1.

References
Add a Paper

Herrmann M, Bara JJ, Sprecher CM, Menzel U, Jalowiec JM, Osinga R, Scherberich A, Alini M, Verrier S

Pericyte plasticity - comparative investigation of the angiogenic and multilineage potential of pericytes from different human tissues

Eur Cell Mater

Volume: 31 (2016) Page: 236-249 PMID: 27062725

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Availability: In stock

Price: $225.00
Size: 10 ug
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