TGIF antibody (10R-1362)

Mouse monoclonal TGIF antibody

Synonyms Monoclonal TGIF antibody, Anti-TGIF antibody, MGC39747 antibody, HPE4 antibody, MGC5066 antibody, TGFB-induced factor homeobox 1 antibody, Anti TGIF antibody, MGC39747 antibody, MGC5066 antibody, HPE4 antibody, TGIF antibody, TGFB-induced factor homeobox 1 antibody
Specificity Human TGIF
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen TGIF antibody was raised in mouse using recombinant Tgfb-Induced Factor (Tale Family Homeobox)

Images

Immunofluorescent staining using TGIF antibody (10R-1362)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TGIF antibody.

Specifications

Host Mouse
Clone 946C1a
Isotype IgG1
Method of Purification TGIF antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TGIFantibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.

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Images

  • Immunofluorescent staining using TGIF antibody (10R-1362) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-TGIF antibody.
  • Western Blot analysis of immunized recombinant protein using TGIF antibody (10R-1362)

Availability: In stock

Price: $190.00
Size: 50 ug
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