Thioredoxin 2 protein (30R-1169)

Purified recombinant Human Thioredoxin 2 protein

Synonyms Thioredoxin 2, MTRX protein, MT-TRX protein, Thioredoxin2 protein, TXN 2 protein, Thioredoxin mitochondrial protein, Thioredoxin-2, Thioredoxin 2 protein, thioredoxin 2 precursor protein, mitochondrial thioredoxin protein, Thioredoxin -2, TRX2 protein, TRX 2 protein, Thioredoxin -2 protein, Thioredoxin 2, TXN2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction cocktail in a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin-2 into reaction cocktail and mix immediately. 3. Incubate at 25 DEG C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.

Images

Coomassie Blue stained SDS-PAGE of Thioredoxin 2 protein (30R-1169)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 60-166 amino acids: MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
Expression System E.coli
Purity > 95% pure
Molecular Weight 11 kDa (108 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin 2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin 2 was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Thioredoxin 2 protein (30R-1169) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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