Thyroxine ELISA Kit (55R-FT4000)

ELISA kit for detection of Thyroxine in the research laboratory

Synonyms Free T4 ELISA Kit
Applications ELISA
Assay Information The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient’s serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potentia

General Information

Biological Significance The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a very small fraction of the circulating hormone is free (unbound) - T4 0.03%.

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Thyroxine ELISA Kit Protocol

Added: Jun 22, 2015


Availability: In stock

Price: $215.00
Size: 96 wells
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