TMB Membrane Substrate (42-TB07)

High sensitivity HRP membrane substrate (containing TMB)


Purity Highly pure
Form & Buffer Supplied as a liquid.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user. The product should be allowed to equilibrate to room temperature (25C) prior to use. For use, completely cover the membrane surface with substrate. The substrate system will react with sites on the membrane containing peroxidase producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes to intense which may result in diminished contrast between the positive and background staining. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.

Storage & Safety

Storage Store at 4 deg C.

General Information

Biological Significance This HRP Membrane Substrate is a ready to use solution containing 3,3?,5,5?- tetramethylbenzidine in a mildly acidic buffer. Unreacted substrate should be faint yellow to pink in appearance. When this substrate system is reacted with peroxidase, an insoluble permanent dark blue reaction product is obtained. This substrate is not recommended for microwell or immunohistochemical applications.

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Availability: In stock

Price: $88.00
Size: 100 ml
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