TMB Substrate (85R-117)

High sensitivity TMB Substrate ready to use in immunoassays


Form & Buffer Supplied as a liquid.

Storage & Safety

Storage Store at 4 deg C.

General Information

Biological Significance TMB Substrate for ELISA is a peroxidase substrate containing 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide in an aqueous buffer formulation. This product is supplied as a one component ready-to-use reagent. Intact substrate is clear colorless to slightly yellowish in appearance. Reaction with peroxidase results in converting substrate into soluble blue (~ 650 nm) product that after stopping with strong acid further turns to super-oxidized soluble yellow product. Yellow product can be measured at 450 nm (measuring at 450 nm vs. 620-650 nm reference wavelength will additionally improve readout in ELISA).

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Availability: In stock

Price: $95.00
Size: 30 ml
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