TMEFF2 antibody (10R-1113)

Mouse monoclonal TMEFF2 antibody

Synonyms Monoclonal TMEFF2 antibody, Anti-TMEFF2 antibody, transmembrane protein TENB2 antibody, TR antibody, TMEFF-2, transmembrane protein with EGF-like and twofollistatin-like domains 2 antibody, tomoregulin antibody, HPP1 antibody, TPEF antibody, cancer/testis antigen family 120 member 2 antibody, TMEFF 2, TMEFF 2 antibody, CT120.2 antibody, TMEFF2, TMEFF-2 antibody, TENB2 antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, WB
Immunogen TMEFF2 antibody was raised in mouse using recombinant human TMEFF2 (41-150aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of TMEFF2 antibody (10R-1113)

Cell lysates of Mouse brain and HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TMEFF2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone J4B6
Isotype IgG2b kappa
Source Anti-human TMEFF2 antibody, clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.
Method of Purification TMEFF2 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance TMEFF2 (transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor?like domain, had limited normal tissue distribution and was highly overexpressed in prostate cancer. The shedded form up-regulates cancer cell proliferation, probably by promoting ERk1/2 phosphorylation. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region.

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Images

  • Western Blot analysis of TMEFF2 antibody (10R-1113) | Cell lysates of Mouse brain and HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TMEFF2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $295.00
Size: 100 ul
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