TPK1 protein (His tag) (80R-2159)
Purified recombinant Human TPK1 protein (His tag)
|Synonyms||Thiamin pyrophosphokinase 1 protein, TPK 1 protein, HTPK1 protein, PP20 protein, TPK-1, TPK 1, TPK-1 protein, TPK1|
Coomassie Blue stained SDS-PAGE of TPK1 protein (His tag) (80R-2159)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS|
|Purity||> 90% pure|
|Molecular Weight||29.8 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||TPK1 catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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