Transferrin protein (FITC) (65C-CH0929)
Transferrin protein (FITC) conjugate
|Synonyms||Apotransferrin protein, beta 1 metal binding globulin protein, DKFZp781D0156 protein, PRO1400 protein, PRO1557 protein, PRO2086 protein, Serotransferrin precursor protein, Siderophilin protein, TF protein|
Dot Blot analysis using Transferrin protein (FITC) (65C-CH0929)
Human Transferrin Fluorescein Load: 3-fold serial dilution starting at 200 ng. Block for 30 min at RT.
|Method of Purification||Transferrin protein (FITC) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.|
|Form & Buffer||Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA and 0.01% NaN3. Immunoglobulin and protease free.|
Usage & Assay Information
|Usage Recommendations||Optimal conditions to be determined by end user|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites.|
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