TRPT1 protein (His tag) (80R-2069)

Recombinant human TRPT1 protein (His tag)

Synonyms tRNA phosphotransferase 1 protein, TRPT-1 protein, tRNA 2'-phosphotransferase 1 protein, MGC11134 protein, TRPT 1, TRPT 1 protein, TRPT-1, TRPT1
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of TRPT1 protein (His tag) (80R-2069)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-253 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ
Expression System E.coli
Purity > 95% pure
Molecular Weight 30.3 kDa (277aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of TRPT1 protein (His tag) (80R-2069) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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