TUBB2b antibody (10R-1184)

Mouse monoclonal TUBB2b antibody

Synonyms Monoclonal TUBB2b antibody, Anti-TUBB2b antibody, TUBBb 2 antibody, TUBBb 2, MGC8685 antibody, TUBB2b, FLJ98847 antibody, bA506K6.1 antibody, tubulin beta 2B antibody, Tubulin beta-2B chain antibody, DKFZp566F223 antibody, TUBBb-2, TUBBb-2 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen TUBB2b antibody was raised in mouse using recombinant human TUBB2b (1-445aa) purified from E. coli as the immunogen.

Images

Immunofluorescent staining using TUBB2b antibody (10R-1184)

Immunofluorescenitrocellulosee of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).

Specifications

Host Mouse
Clone AT5B3
Isotype IgG2a kappa
Source Anti-human TUBB2b antibody, clone AT5B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TUBB2b protein.
Method of Purification TUBB2b antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Multiple beta tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mainly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. Also, TUBB2B is a new critical gene involved in neuronal migration. It is present in the nuclei and nucleoplasm, Defects in this gene are a cause of asymmetric polymicrogyria.

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Images

  • Immunofluorescent staining using TUBB2b antibody (10R-1184) | Immunofluorescenitrocellulosee of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).
  • Western Blot analysis of TUBB2b antibody (10R-1184) | Cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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