TUBB3 protein (His tag) (80R-2700)
Purified recombinant TUBB3 protein (His tag)
|Synonyms||Tubulin protein, beta 3 class III protein, beta-4 protein, CDCBM protein, CFEOM3A protein, TUBB4 protein, TUBB3, TUBB 3, TUBB-3, TUBB 3 protein, TUBB-3 protein|
SDS-PAGE analysis of TUBB3 protein (His tag) (80R-2700)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-450 aa: MGSSHHHHHH SSGLVPRGSH MGSMREIVHI QAGQCGNQIG AKFWEVISDE HGIDPSGNYV GDSDLQLERI SVYYNEASSH KYVPRAILVD LEPGTMDSVR SGAFGHLFRP DNFIFGQSGA GNNWAKGHYT EGAELVDSVL DVVRKECENC DCLQGFQLTH SLGGGTGSGM GTLLISKVRE EYPDRIMNTF SVVPSPKVSD TVVEPYNATL SIHQLVENTD ETYCIDNEAL YDICFRTLKL ATPTYGDLNH LVSATMSGVT TSLRFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTARG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVATVF RGRMSMKEVD EQMLAIQSKN SSYFVEWIPN NVKVAVCDIP PRGLKMSSTF IGNSTAIQEL FKRISEQFTA MFRRKAFLHW YTGEGMDEME FTEAESNMND LVSEYQQYQD ATAEEEGEMY EDDEEESEAQ GPK|
|Purity||> 90% pure|
|Molecular Weight||52.8 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles|
|Biological Significance||Tubulin, beta 3 class III, also known as TUBB3, is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Recombinant human TUBB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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