TUG antibody (10R-1832)

Mouse monoclonal TUG antibody

Synonyms Monoclonal TUG antibody, Anti-TUG antibody
Cross Reactivity Mouse
Applications ELISA, WB
Immunogen TUG antibody was raised in Mouse using a purified recombinant fragment of TUG expressed in E. coli as the immunogen.

Images

Western Blot analysis using TUG antibody (10R-1832)

Western Blot showing TUG antibody used against NIH/3T3 cell lysate.

Specifications

Host Mouse
Clone 4A11A6G11
Isotype IgG1
Form & Buffer Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.

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Images

  • Western Blot analysis using TUG antibody (10R-1832) | Western Blot showing TUG antibody used against NIH/3T3 cell lysate.

Availability: In stock

Price: $295.00
Size: 100 ul
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