TWF1 protein (His tag) (80R-2064)

Recombinant human TWF1 protein (His tag)

Synonyms TWF-1, PTK9 protein, Twinfilin-1 protein, TWF-1 protein, TWF 1, TWF 1 protein, TWF1
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of TWF1 protein (His tag) (80R-2064)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-252 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD
Expression System E.coli
Purity > 80% pure
Molecular Weight 31.5 kDa (277aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of ~20 residues. The two ADF-H domains are ~20% homologous to ADF/cofilin and to each other. Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of TWF1 protein (His tag) (80R-2064) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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