TXNRD1 protein (His tag) (80R-1334)

Purified recombinant Human TXNRD1 protein

Synonyms MGC9145 protein, Oxidoreductase protein, MGC9145 protein, Thioredoxin reductase TR1 protein, Thioredoxin reductase GRIM 12 protein, TRXR1 protein, TXNRD-1, TXNR protein, TXNRD 1 protein, TXNRD 1., TXNRD-1 protein, GRIM12 protein, TRXR 1 protein, TXNRD 1, KDRF protein, Thioredoxin reductase 1 cytoplasmic protein, TXNRD1, Thioredoxin reductase 1 protein, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein protein, GRIM-12 protein, TR 1 protein, KM 102 derived reductase like factor protein, TR1 protein, GRIM 12 protein, TR protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 0.7ml reaction mixture into a suitable container: The final concentrations are 100mM potassium phosphate, 10mM EDTA, 0.2mM beta-NADPH, 0.05% BSA, 0.014% (w/v) thioredoxin, 5mM DTNB, 5ug, human TRXNRD1. 2. Equilibrate to 25 DEG C and monitor the A412nm until the value is constant using a spectrophotometer. 3. Add 35ul of 100mM DTNB into reaction mixture and mix immediately. 4. Record the increase in A412nm for 2 minutes.

Images

Coomassie Blue stained SDS-PAGE of TXNRD1 protein (His tag) (80R-1334)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 161-647 amino acids: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC
Expression System E.coli
Purity > 95% pure
Molecular Weight 55.7 kDa (508aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH7.4, containing 10% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 0.48-0.6 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of TXNRD1 protein (His tag) (80R-1334) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
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