Ubc9 protein (30R-1043)

Purified recombinant Human Ubc9 protein

Synonyms EC 6.3.2 protein, UBE 2I protein, Ubiquitin conjugating enzyme UbcE2A protein, SUMO-conjugating enzyme UBC9 protein, Ubiquitin like protein SUMO 1 conjugating enzyme protein, Ubiquitin protein ligase E2I protein, Ubiquitin carrier protein 9 protein, UBCE 9 protein, SUMO1 protein ligase protein, Ubiquitin like protein SUMO1 conjugating enzyme protein, SUMO protein ligase protein, Ubiquitin protein ligase I protein, Human ubiquitin conjugating enzyme 9 protein, UBC9 homolog protein, p18 protein, SUMO 1 conjugating enzyme protein, , Ubiquitin conjugating enzyme E2I protein, UBCE9 protein, SUMO 1 protein ligase protein, Ubiquitin-conjugating enzyme E2I C358B7.1 protein, Ubiquitin-protein ligase I protein, UBC 9 protein, Ubiquitin carrier protein I protein, Ubiquitin carrier protein protein, SUMO1 conjugating enzyme protein, Ubiquitin conjugating enzyme 9 protein, p18 protein, Homologous to yeast UBC9 protein, UBE2I protein, Ubc9 protein, UBC9 protein, SUMO-protein ligase protein, SUMO conjugating enzyme UBC9 protein, Ubiquitin-conjugating enzyme E2 I protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Ubc9 protein (30R-1043)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS
Expression System E.coli
Purity > 95% pure
Molecular Weight 18 kDa (158 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 50mM HEPES, pH7.5, containing 150mM NaCl, 1mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.

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Images

  • Coomassie Blue stained SDS-PAGE of Ubc9 protein (30R-1043) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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