UBE2B protein (His tag) (80R-1333)
Purified recombinant Human UBE2B protein
|Synonyms||Ubiquitin-conjugating enzyme E2B hHR6B protein, Ubiquitin carrier protein B protein, UBE2B, Ubiquitin conjugating enzyme E2 B protein, RAD6B protein, UBC2 protein, HR6B protein, UBEB-2 protein, HHR6B protein, Ubiquitin-conjugating enzyme E2 17 kDa., RAD6 homolog B protein, Ubiquitin protein ligase B protein, UBEB 2, UBEB-2, E2-17kDa protein, UBEB 2 protein|
Coomassie Blue stained SDS-PAGE of UBE2B protein (His tag) (80R-1333)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-152 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS|
|Purity||> 90% pure|
|Molecular Weight||21.4 kDa (188aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It is required for postreplication repair of UV-damaged DNA. It may be involved in neurite outgrowth. Recombinant human UBE2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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