UBE2D2 protein (His tag) (80R-1901)
Purified recombinant UBE2D2 protein
|Synonyms||UBED-2, UBC4/5 protein, UBC4 protein, UBED-2 protein, UBCH5B protein, PUBC1 protein, E2(17)KB2 protein, UBED 2, UBE2D2, UBED 2 protein|
Coomassie Blue stained SDS-PAGE of UBE2D2 protein (His tag) (80R-1901)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-147 amino acids: MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM|
|Purity||> 95% pure|
|Molecular Weight||18.9 kDa (167aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human UBE2D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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