UBE2F protein (His tag) (80R-2346)

Purified recombinant Human UBE2F Protein (His tag)

Synonyms NEDD8-conjugating enzyme UBE2F protein, UBEF-2 protein, NCE2 protein, UBEF 2 protein, UBE2F, UBEF-2, UBEF 2
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UBE2F protein (His tag) (80R-2346)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR
Expression System E.coli
Purity > 95% pure
Molecular Weight 23.7 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer, pH 8.0, 30% glycerol, 1mM DTT, 200mM NaCl
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UBE2F, also Known as Ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of UBE2F protein (His tag) (80R-2346) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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