UBE2T protein (His tag) (80R-1294)

Purified recombinant Human UBE2T protein

Synonyms HSPC150 protein similar to ubiquitin conjugating enzyme protein, HSPC150 protein, UBET 2, UBET-2, Ubiquitin-conjugating enzyme E2T Cell proliferation inducing gene 50 protein protein, UBET-2 protein, UBE2T, PIG50 protein, , Ubiquitin conjugating enzyme E2T protein, Ubiquitin protein ligase T protein, UBET 2 protein, HSPC150 protein similar to ubiquitin-conjugating enzyme protein, Ubiquitin conjugating enzyme protein, UBE2T protein, Ubiquitin carrier protein T protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UBE2T protein (His tag) (80R-1294)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-197 amino acids: MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH
Expression System E.coli
Purity > 95% pure
Molecular Weight 23.6 kDa (205aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerola and 1mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, UBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. Recombinant UBE2T protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of UBE2T protein (His tag) (80R-1294) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
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