UBE2W protein (His tag) (80R-1835)

Purified recombinant UBE2W protein

Synonyms FLJ11011 protein, Probable ubiquitin-conjugating enzyme E2 W protein, UBEW-2, UBEW 2, UBEW 2 protein, UBEW-2 protein, UBE2W, hUBC-16 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UBE2W protein (His tag) (80R-1835)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-151 amino acids: MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
Expression System E.coli
Purity > 90% pure
Molecular Weight 19.5 kDa (171aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 0.1M NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is widely expressed, especially at highest levels in testis. Recombinant human UBE2W protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of UBE2W protein (His tag) (80R-1835) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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