UCHL5 protein (His tag) (80R-1826)
Purified recombinant UCHL5 protein
|Synonyms||UCHL 5, UCHL-5, Ubiquitin carboxyl-terminal hydrolase isozyme L5 protein, Ubiquitin carboxyl-terminal hydrolase isozyme L5 protein, INO80R protein, UCHL 5 protein, AD-019 protein, UCHL-5 protein, UCH-L5 protein, INO80 complex subunit R protein, UCH37 protein, UCHL5, CGI-70 protein|
Coomassie Blue stained SDS-PAGE of UCHL5 protein (His tag) (80R-1826)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-329 amino acids: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK|
|Purity||> 90% pure|
|Molecular Weight||39.7 kDa (349aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF and 2mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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