UGDH protein (His tag) (80R-1374)
Purified recombinant Human UGDH protein
|Synonyms||UGD protein, GDH protein, UDPGDH protein, UDP-GlcDH protein, UDP-glucose 6-dehydrogenase, UDP-glucose dehydrogenase protein|
Coomassie Blue stained SDS-PAGE of UGDH protein (His tag) (80R-1374)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-494 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV|
|Purity||> 95% pure|
|Molecular Weight||59.5 kDa (533aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.|
Usage & Assay Information
|Bioactivity||Specific activity: > 0.1 unit/ml. One unit will oxidize 1.0 umol of UDP-glucose to UDP-glucuronic acid per minute at pH 8.0 at 25C|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human UGDH protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques|
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