UGDH protein (His tag) (80R-1374)

Purified recombinant Human UGDH protein

Synonyms UGD protein, GDH protein, UDPGDH protein, UDP-GlcDH protein, UDP-glucose 6-dehydrogenase, UDP-glucose dehydrogenase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UGDH protein (His tag) (80R-1374)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-494 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
Expression System E.coli
Purity > 95% pure
Molecular Weight 59.5 kDa (533aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity: > 0.1 unit/ml. One unit will oxidize 1.0 umol of UDP-glucose to UDP-glucuronic acid per minute at pH 8.0 at 25C

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human UGDH protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of UGDH protein (His tag) (80R-1374) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 50 ug
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