UNC119B protein (His tag) (80R-2140)

Recombinant human UNC119B protein (His tag)

Synonyms UNCB-119, Protein unc-119 homolog B protein, POC7B protein, UNCB 119 protein, UNCB 119, UNC119B, UNCB-119 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UNC119B protein (His tag) (80R-2140)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-251 amino acids: MGSSHHHHHH SSGLVPRGSH MSGSNPKAAA AASAAGPGGL VAGKEEKKKA GGGVLNRLKA RRQAPHHAAD DGVGAAVTEQ ELLALDTIRP EHVLRLSRVT ENYLCKPEDN IYSIDFTRFK IRDLETGTVL FEIAKPCVSD QEEDEEEGGG DVDISAGRFV RYQFTPAFLR LRTVGATVEF TVGDKPVSNF RMIERHYFRE HLLKNFDFDF GFCIPSSRNT CEHIYEFPQL SEDVIRLMIE NPYETRSDSF YFVDNKLIMH NKADYAYNGG Q
Expression System E.coli
Purity > 90% pure
Molecular Weight 30.3 kDa (271aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance UCN119B (Protein unc-119 homolog B) belongs to the PDE6D/unc-119 family. This protein is enriched in the photoreceptors of the retina. It shares strong homology with the C. elegans unc119 protein and it can functionally complement the C. elegans unc119 mutation gene. The mammalian multisubunit photoreceptor cGMP phosphodiesterase PDE alpha beta gamma 2 (PDE6 family) is a peripherally membrane-associated enzyme. Recombinant human UNC119B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of UNC119B protein (His tag) (80R-2140) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 100 ug
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