URM1 protein (His tag) (80R-1752)
Purified recombinant Human URM1 protein
|Synonyms||URM 1 protein, URM1, C9orf74 protein, MGC2668 protein, Ubiquitin-related modifier 1 homolog protein, URM-1, URM-1 protein, URM 1, RP11-339B21.4 protein|
Coomassie Blue stained SDS-PAGE of URM1 protein (His tag) (80R-1752)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-101 amino acids: MGSSHHHHHH SSGLVPRGSH MAAPLSVEVE FGGGAELLFD GIKKHRVTLP GQEEPWDIRN LLIWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SVLFISTLHG G|
|Purity||> 95% pure|
|Molecular Weight||13.5 kDa (121 aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||URM1 is ubiquitin-related modifier 1 homolog protein that primarily functions in the post-translational modification of proteins by way of the urmylation pathway. In studies with Saccharomyces cerevisiae, it has been found that Urm1 covalently binds to its E1 activating enzyme, Uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1). It is hypothesized that this complex may then play a role in the oxidative-stress response in mammals. Recombinant human URM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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